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Cell Signaling Technology Inc opa1

COPN restores mitochondrial quality control and interrupts the ROS‒cGAS‒inflammation axis in OGD/R-treated R28 cells. a , TEM images showing changes in the mitochondrial ultrastructure of R28 cells subjected to various treatments (PBS, PDNs, COPN-L, and COPN-H) after OGD/R. Scale bar: 500 nm b , Quantitative analyses of mitochondrial length and number in R28 cells as indicated in a . c , Representative fluorescence images showing JC-1 staining of the mitochondrial Δψm: aggregates (red) indicate healthy mitochondria, whereas monomers (green) represent depolarized mitochondria. Nuclei were stained with DAPI (blue). Scale bar: 50 μm d , Intracellular ATP content assay revealing improved energy production after COPN treatment under OGD/R stress conditions. e , mRNA expression analysis of mitochondrial dynamic regulatory genes <t>(Opa1,</t> Mfn2, Drp1, and Fis1) and mitochondrial DNA transcription levels (mt-ND1 and mt-COX1). f , Quantitative analysis of the JC-1 fluorescence ratio (aggregates/monomers) and mitochondrial ROS levels (MitoSOX staining). g–h , Western blot analysis of proteins involved in mitochondrial autophagy (Pink1, Parkin, and P62) (g) and mitochondrial fusion/fission (Opa1, Mfn2, Mfn1, Drp1, and Fis1) (h) . i , Confocal fluorescence microscopy images of mitochondria (green, MitoTracker) and lysosomes (red, LysoTracker) in R28 cells after different treatments. Nuclei were stained with Hoechst (blue). Scale bar: 50 μm ∗ ∗∗∗P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Opa1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-opa1/OPA1+Rabbit+mAb/pmc13049686-160-30-31
Average 96 stars, based on 412 article reviews

opa1 - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation"

Article Title: A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation

Journal: Materials Today Bio

doi: 10.1016/j.mtbio.2026.102974

Figure Legend Snippet: COPN restores mitochondrial quality control and interrupts the ROS‒cGAS‒inflammation axis in OGD/R-treated R28 cells. a , TEM images showing changes in the mitochondrial ultrastructure of R28 cells subjected to various treatments (PBS, PDNs, COPN-L, and COPN-H) after OGD/R. Scale bar: 500 nm b , Quantitative analyses of mitochondrial length and number in R28 cells as indicated in a . c , Representative fluorescence images showing JC-1 staining of the mitochondrial Δψm: aggregates (red) indicate healthy mitochondria, whereas monomers (green) represent depolarized mitochondria. Nuclei were stained with DAPI (blue). Scale bar: 50 μm d , Intracellular ATP content assay revealing improved energy production after COPN treatment under OGD/R stress conditions. e , mRNA expression analysis of mitochondrial dynamic regulatory genes (Opa1, Mfn2, Drp1, and Fis1) and mitochondrial DNA transcription levels (mt-ND1 and mt-COX1). f , Quantitative analysis of the JC-1 fluorescence ratio (aggregates/monomers) and mitochondrial ROS levels (MitoSOX staining). g–h , Western blot analysis of proteins involved in mitochondrial autophagy (Pink1, Parkin, and P62) (g) and mitochondrial fusion/fission (Opa1, Mfn2, Mfn1, Drp1, and Fis1) (h) . i , Confocal fluorescence microscopy images of mitochondria (green, MitoTracker) and lysosomes (red, LysoTracker) in R28 cells after different treatments. Nuclei were stained with Hoechst (blue). Scale bar: 50 μm ∗ ∗∗∗P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Techniques Used: Control, Fluorescence, Staining, Expressing, Western Blot, Microscopy

other:

Article Title: The second mitochondrial activator of caspases (SMAC) regulates growth, inflammation and mitochondrial integrity in cancer cells.
Article Snippet: Antibodies The following primary antibodies were used: anti-γH2AX (#9718S, Cell Signaling), anti-GAPDH (MAB374, Millipore), anti-cleaved caspase-3 (#9661, Cell Signaling Technology), anti-caspase-3 (#9662, Cell Signaling Technology), anti-SMAC (#2954 and #15108, Cell Signaling Technology), anticytochrome c (#11940 and #12963, Cell Signaling Technology), antiphospho-STING (#19781S, Cell Signaling Technology), anti-phospho-IRF3 (#ab76493, Abcam), anti-TFAM (#8076S, Cell Signaling Technology), antidsDNA (#ab27156, Abcam) anti-biotin (#SAB4200680-100UL, SigmaAldrich) anti-LAMP2 (#49067T, Cell Signaling Technology), anti-BiP (#3177T, Cell Signaling Technology), anti-COX IV-Alexa Flour 488 conjugate A Lung adenocarcinoma Colon-rectum adenocarcinmoa Cell Death & Differentiation (#4853S, Cell Signaling Technology), anti-OPA1 (#67589S, Cell Signaling Technology), anti MIC10 (#31561-1-AP, Proteintech) and anti-TOM20 (#42406, Cell Signaling Technology).

Article Title: Protective role of fatty acid oxidation against epithelial barrier dysfunction in allergic asthma
Article Snippet: Primary antibodies used were anti-CPT1A (Abcam, UK, ab234111), anti-DRP1 (Cell Signaling Technology, CST, USA, 8570), anti-FIS1 (CST, 32525), anti-OPA1 (CST, 80471), anti-MFN2 (CST, 9482), anti-NLRP3 (CST, 15101), Anti-Caspase-1 (Abcam, ab179515), anti-p-AMPK (CST, 50081), anti-CD36 (CST, 14347), anti-GLUT (CST, 12939), anti-LDHA (CST, 2012), anti-ZO-1 (Abcam, ab276131), anti-occludin (Abcam, ab216327), and anti-β-actin (CST, 3700) at a dilution ratio of 1:1000.

Article Title: Effect of Tofogliflozin on Skeletal Muscle Mitochondrial Function in Male Diabetic Mice With Muscle Atrophy
Article Snippet: The primary antibodies used were rabbit anti-AMPKα (adenosine monophosphate [AMP]-activated protein kinase α, AMPKα; Cell Signaling, No. 2532; RRID: AB_330331 ), rabbit anti-phospho-AMPKα (Thr172) (Cell Signaling, No. 2531; RRID: AB_330330 ), rabbit anti-Akt (Protein kinase B, Akt; Cell Signaling, No. 4691; RRID: AB_915783 ), rabbit anti-phospho-Akt (Ser473) (Cell Signaling, No. 9271; RRID: AB_329825 ), rabbit anti-S6 (ribosomal protein S6, S6; Cell Signaling, No. 2217; RRID: AB_331355 ), rabbit anti-phospho-S6 (Ser235/236) (Cell Signaling, No. 2211; RRID: AB_331679 ), rabbit anti-MuRF1/2/3 (muscle RING finger proteins 1-3, MuRF1/2/3; Abcam, ab172479; RRID: AB_3712411 ), rabbit anti-myostatin (myostatin, MSTN; Abcam, ab71808; RRID: AB_1268982 ), rabbit anti-SIRT1 (sirtuin 1, SIRT1; SirT1 (D1D7) Rabbit mAb, Cell Signaling, No. 9475; RRID: AB_2617130 ), rabbit anti-PGC1α (peroxisome proliferator-activated receptor γ coactivator 1-α, PGC1α; Anti-PGC1α-N-terminus, Abcam, ab191838; RRID: AB_2721267 ), rabbit anti-OPA1 (optic atrophy 1, OPA1; Cell Signaling, No. 80471; RRID: AB_2734117 ), rabbit anti-DRP1 (dynamin-related protein 1, DRP1; Cell Signaling, No. 8570; RRID: AB_10950498 ), rabbit anti-GDF15 (growth differentiation factor-15, GDF15; Abcam, ab105738; RRID: AB_10937795 ), and mouse anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase, GAPDH; Abcam, ab9484; RRID: AB_307274 ).

Article Title: A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injury
Article Snippet: Anti-OPA1 antibody , Cell Signaling Technology , Cat#80471; RRID:AB_2734117.

Western Blot:

Article Title: Early mitophagy activation by Urolithin A prevents, but late activation does not reverse, age-related cognitive impairment.
Article Snippet: The fluo- rescent dyes used were: CM-H2DCFDA (catalog number C6827, Thermo Fisher Scientific), TPE-MI ( Tetraphenylethene maleimide)(Catalog number: HY-143218 Med Chem Express). .. The primary antibodies used were: mouse anti-β-actin (1:1000, sc-47778, Santa Cruz Biotechnology, Inc.), rabbit anti-GAPDH (1:1000, sc-25778, Santa Cruz Biotechnology, Inc.), rabbit anti-GAPDH (1:1000, sc25778, Santa Cruz Biotechnology, Inc.), mouse anti-synaptophysin (1:1000, sc-17750, Santa Cruz Biotechnology, Inc.), mouse anti-PSD95 (1:500, sc-32290, Santa Cruz Biotechnology, Inc.), mouse antiGluA1(1:100, sc13152, Santa Cruz Biotechnology,Inc.)., mouse anti-Total OXPHOS Human WB Antibody Cocktail (1:1000, ab110411, Abcam, Inc.), rabbit anti-Opa1 (1:1000, mAb 80471, Cell Signaling Technologies), mouse anti-Mfn1 (1:1000. sc-166644, Santa Cruz Biotechnology, Inc), rabbit antiMfn2 (1:1000, mAb 11925, Cell Signaling Technologies), rabbit anti-pDrp1 (1:1000 mAb 6319; mb 4494, Cell Signaling Technolo-gies), mouse anti-Drp1 (1:1000, sc-271583, Santa Cruz Biotechnology, Inc), rabbit anti-FUNDC1 (1:1000, PA5-77785, Invitrogen), mouse anti-TBK1 (sc-52957, Santa Cruz Biotechnology, Inc) mouse anti-PINK1 (1:1000, sc-517353, Santa Cruz Biotechnology, Inc.), mouse anti-Parkin (1:1000, sc-32282, Santa Cruz Biotechnology, Inc), rabbit anti-Nrf2 (1:1000, sc722, Santa Cruz Biotechnology, Inc.), mouse anti-PGC-1α (1:1000, sc-517380, Santa Cruz Biotechnology, Inc.), rabbit anti-p62 (1:1000, mAb 8025, Cell Signaling Technologies), rabbit anti-OPTN (1:1000, mAb 58981, Cell Signaling Technologies), rabbit anti-LC3 (1:1000, mAb 3868, Cell Signaling Technologies), rabbit anti-BNIP/NIX (1:1000, mAb 12396, Cell Signaling Technologies), rabbit antiSesn2 (1:1000, mAb 8487, Cell Signaling Technologies), rabbit anti-human tau (1:1000, 2024-10-31, Dako), mouse anti-tau PHF-1 (phosphorylated at Ser396 and Ser394) was a gift by Dr. Peter Davies (Department of Pathology, Albert Einstein College of Medicine, NY, USA. ..

Incubation:

Article Title: HUWE1 regulates mitophagy to protect dopaminergic neurons from 6-OHDA- and MPP⁺-induced neurotoxicity.
Article Snippet: .. The membranes were blocked with 5% non-fat milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-HUWE1 (1:1,000, #9482, Cell Signaling Technology, MA, USA), anti-TOMM20 (1:1,000, #42406, CST), anti-β-actin (1:1,000, sc-47778, Santa Cruz, CA, USA), anti-GAPDH (1:1,000, sc-32233, Santa Cruz), anti-Myc (1:1,000, #9B11, CST), anti-COX IV (1:1,000, #3E11, CST), anti-LC3B (1:1,000, #2775, CST), anti-Bcl-2 (1:1,000, sc-783, Santa Cruz), anti-Bax (1:1,000, sc-6236, Santa Cruz), anti-Cleaved caspase-3 (1:1,000, #9664, CST), anti-DRP1 (1:1,000, #8570, CST), anti-MFN2 (1:1,000, #9482, CST), anti-OPA1 (1:1,000, #80471, CST), anti-p-ULK1 (1:1,000, #5869, CST), anti-ULK1 (1:1,000, A8529, ABclonal, MA, USA), anti-AMBRA1 (1:1,000, A1083, ABclonal), and antiUbiquitin (1:1,000, #39269, CST). ..

Article Title: HUWE1 regulates mitophagy to protect dopaminergic neurons from 6-OHDA- and MPP⁺-induced neurotoxicity
Article Snippet: .. The membranes were blocked with 5% non-fat milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-HUWE1 (1:1,000, #9482, Cell Signaling Technology, MA, USA), anti-TOMM20 (1:1,000, #42,406, CST), anti-β-actin (1:1,000, sc-47778, Santa Cruz, CA, USA), anti-GAPDH (1:1,000, sc-32233, Santa Cruz), anti-Myc (1:1,000, #9B11, CST), anti-COX IV (1:1,000, #3E11, CST), anti-LC3B (1:1,000, #2775, CST), anti-Bcl-2 (1:1,000, sc-783, Santa Cruz), anti-Bax (1:1,000, sc-6236, Santa Cruz), anti-Cleaved caspase-3 (1:1,000, #9664, CST), anti-DRP1 (1:1,000, #8570, CST), anti-MFN2 (1:1,000, #9482, CST), anti-OPA1 (1:1,000, #80,471, CST), anti-p-ULK1 (1:1,000, #5869, CST), anti-ULK1 (1:1,000, A8529, ABclonal, MA, USA), anti-AMBRA1 (1:1,000, A1083, ABclonal), and anti-Ubiquitin (1:1,000, #39269, CST). .. After washing, the membranes were incubated with HRP-conjugated secondary antibodies (anti-mouse or anti-rabbit, 1:5,000, Santa Cruz) for 1 h at room temperature.

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